| 제목 |
Hybrid regenerative effects of 3D bioprinting with stem cell in rotator cuff tear in rabbit model |
| 소속 |
Daegu Catholic University Medical Center, Department of Rehabilitation Medicine1, Severance Hospital, Department of Rehabilitation Medicine2, Daegu Catholic University Medical Center, Department of Anatomy3, Others, POSTECH, Department of Creative IT Engineering and IBIO4 |
| 저자 |
Sang Chul Lee2, Dong Rak Kwon1*†, Gi Young Park1, Yong Suk Moon3, Jinah Jang4, In Ho Woo1 |
Objective. The aim of this study was to compare the regenerative effects of implantation with three-dimensional bioprinting of multilayered construct containing umbilical cord blood (UCB)-derived mesenchymal stem cell (MSC) and ultrasound (US)-guided injection with human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs).
Methods. Rabbits (n=18) were allocated into 3 groups. After a 5-mm sized full-thickness rotator cuff tendon tear (FTRCTT) just proximal to the insertion site on the supraspinatus tendon was created by excision, the wound was immediately closed by subcutaneous and skin sutures. Two injections (0.2 mL normal saline, G1-SAL; 0.2 mL UCB-MSCs, G2-MSC) were performed into FTRCTT under US guidance. 3D construct containing UCB-derived MSC was implanted into the FTRCTT (0.2mL UCB-MSCs+3D construct, G3-MSC+3D). Gross morphologic changes and histologic examination were performed on all rabbits after sacrifice. Motion analysis was also performed.
Results. The gross morphologic mean tendon tear size in G3-MSC+3D and G2-MSC was significant smaller than that of G1-SAL (p<.05). There was no significant difference in tendon tear size between G2-MSC and G3-MSC+3D. However there were differences in the degree of tendon recovery among three groups (p<.05). Complete healing(CH) was observed in two rabbits (33%) and partial thickness tear(PTT) was observed in four rabbits (67%) in G3-MSC+3D, CH was observed in one rabbit (16.7%), PTT was observed in three rabbits (50%) in G2-MSC. In G1-SAL, FTT was observed in every rabbits.
In G3-MSC+3D, regenerative activity, angiogenesis, walking distance, fast walking time, mean walking speed were greater than in the other two groups on histological examination and motion analysis.
Conclusions. Three dimensional bioprinting of multilayered construct implantation with mesenchymal stem cell treatment was more effective than ultrasound guided injection in gross morphological, histological and motion analysis in a rabbit model of traumatic FTRCTT.
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Gross morphological and immunohistochemical findings
Semiquantitative score of histological findings, immunoreactivity of stain
Semiquantitative score of gross morphologic, histological findings, immunoreactivity of stain and motion analysis according to treatment groups at 4 weeks after injection.
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