| 제목 |
Pilot study on the effect of botulinum toxin type A in rats with complex regional pain syndrome |
| 소속 |
St. Paul Hospital, The Catholic University of Korea, Department of Rehabilitation Medicine1 |
| 저자 |
Jihye Park1*, Myung Eun Chung1, Dae Heon Song1† |
Objectives
The application of botulinum toxin type A (BTX-A) has recently been studied in a variety of neuropathic pain syndromes. The aim of this study is to evaluate the effects of BTX-A on neuropathic pain with complex regional pain syndrome (CRPS) through behavioral sensory test.
Methods
Twenty male Sprague-Dawley rats weighting 350 ~ 450g were induced CPRS model by partial sciatic nerve ligation method. 50U/vial of BTX-A (Medytoxin) were reconstructed in adequate volume of 0.9% saline to obtain respective doses. Saline control or BTX-A(20U/kg)* with a volume of 20㎕ were injected with a 27 gauge needle into the plantar surface of the right hindpaw in rats at postoperative day 7 after sciatic nerve ligation. Mechanical allodynia was measured using von Frey filament, and thermal hyperalgesia was measured on a hot plate analgesia meter. Two-way analysis of variance (ANOVA) with repeated measures was used to detect statistical significance.
Results
Three rats without neuropathic pain were excluded and a total of 17 rats were analyzed. The paw withdrawal threshold (PWT) to mechanical stimulation and the paw withdrawal latency (PWL) to thermal stimulation decreased immediately and significantly after partial sciatic nerve ligation. After subcutaneous injection of BTX-A, the PWT to mechanical stimulation was increased and higher than that of the control group (Figure 1, Table 1). However, there was no statistically significant difference between the two groups. The PWL to thermal stimulation gradually increased after injection of BTX-A, but there was no difference compared to control group (Figure 2).
Conclusions
Subcutaneous injection of BTX-A tended to be effective for neuropathic pain caused by CRPS. The impact of BTX-A on CRPS needs to be assessed in future large-scale sample studies. |
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Figure 1. Comparison of paw withdrawal threshold to mechanical stimulation after subcutaneous injection of BTX-A.
Figure 2. Comparison of paw withdrawal latency to thermal stimulation after subcutaneous injection of BTX-A.
Table 1. Effects of BTX-A on pain threshold in CRPS rat model.
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